Ergosterol Analysis Service

With extensive experience and state-of-the-art technologies, Creative Proteomics uses highly sensitive LC-ESI/MS method for the rapid identification and quantification of ergosterol in fungi, which can satisfy the needs of academic and industrial study.

Overview

Ergosterol靶向脂质族学

Ergosterol(ERG)是真菌等血浆和线粒体膜中存在的主要甾醇,以及用于维持膜流动性,渗透性和结构的功能。ERG还形成脂质筏,其富含许多生物学上重要的分子,如排除泵,钠和钾泵,受体和营养转运蛋白。这些微泡是各种细胞过程,应力响应和对环境的适应的核心;维持脂质筏对于细胞正常运作至关重要。它还具有多种在生长调节中的功能,并且是维生素D2的前体。由于其关键功能,独特的结构性,以及特定的生物合成步骤,Ergosterol是大多数临床上抗真菌药的目标。

For the analysis of ergosterol, Creative Proteomics developed a liquid chromatography/mass spectrometry (LC/MS) method to monitor ergosterol biosynthesis. The combination of MS instrument and liquid chromatography (LC) can effectively separate target lipid molecules from complex samples.

我们的Ergosterol Analysis服务的优势

  • 创意蛋白质组学provides comprehensive services from prepare the samples, run the mass spectrometric analysis, process the data, perform statistical analysis, and identify the compounds.
  • 数据分析包括PCA,PLS-DA,PLS-R和单变量统计数据,我们生成了基于统计测试的选择对数据进行分类的脂质清单:F-Test,T-Tests或回归和出口列表进行进一步分析,如此作为富集和Kegg途径分析。
  • We will use our expertise to help you interpret the results and provide you with actionable recommendations on how to utilize these results in your research or business.

我们的Ergosterol Analysis Service的技术过程

The workflow of ergosterol analysis serviceThe workflow of ergosterol analysis service

Structural details:species level
Precision: pmol & mol%
确定的变体:1
Delivery time:2-4周
样本要求

  • 200-350 mg干重/样品
  • A minimum of 6 replicate plates or wells per condition are required.
  • After the sample is collected, it will be stored at -80°C immediately and transported by dry ice.
  • 应用样本标识符清楚地标记管,并包括伴随货物的样本列表的硬拷贝。运输样品时请包括所有可用的样本信息。

Deliverables

  • Experimental procedure
  • 液相色谱和MS的参数
  • Purity analysis report
  • MS原始数据文件和MS数据质量检查
  • Hexylceramide quantitative result data
  • Custom analysis report

With years of experience in lipidomics analysis services,创意蛋白质组学在支持各种脂质的检测和定量方面具有良好的声誉。基于高度稳定,可重复和高度敏感的分离,表征,识别和定量分析系统,与LC-MS / MS相结合,我们提供可靠,快速且经济高效的ERG分析服务。如果您对我们的ERG有疑问有疑问,请contact us.

* Our services can only be used for research purposes and Not for clinical use.

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