NUDT5 Full-Length MS Protein Standard

Protein Name

nudix (nucleoside diphosphate linked moiety X)-type motif 5

Description

NUDT5 Full-Length MS Protein Standard (NP_054861), Labeled with [U-¹³C₆,¹⁵N₄]-L-Arginine and [U-¹³C₆,¹⁵N₂]-L-Lysine, was produced in human 293 cells (HEK293) with fully chemically defined cell culture medium to obtain incorporation efficiency at Creative-Proteomics. This gene belongs to the Nudix (nucleoside diphosphate linked moiety X) hydrolase superfamily. The encoded enzyme catalyzes the hydrolysis of modified nucleoside diphosphates, including ADP-ribose (ADPR) and 8-oxoGua-containing 8-oxo-dADP and 8-oxo-dGDP. Protein-bound ADP ribose can be hazardous to the cell because it can modify some amino acid residues, resulting in the inhibition of ATP-activated potassium channels. 8-oxoGua is an oxidized form of guanine that can potentially alter genetic information by pairing with adenine and cytosine in RNA. Presence of 8-oxoGua in RNA results in formation of abnormal proteins due to translational errors.

Symbol

NUDT5

Synonyms

hYSAH1; YSA1; YSA1H; YSAH1

Accession

NM_014142

Cytogenetic

10p14

Amino Acid Labeled

[U-¹³C₆,¹⁵N₄]-L-Arg and [U-¹³C₆,¹⁵N₂]-L-Lys

Chemical Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Expression Host

Human HEK293 cells

Predicted MW

24.1 kDa

Expression True or False Clone

RC200204

Protein Sequence

>RC200204 representing NM_014142
MESQEPTESSQNGKQYIISEELISEGKWVKLEKTTYMDPTGKTRTWESVKRTTRKEQTADGVAVIPVLQR
TLHYECIVLVKQFRPPMGGYCIEFPAGLIDDGETPEAAALRELEEETGYKGDIAECSPAVCMDPGLSNCT
IHIVTVTINGDDAENARPKPKPGDGEFVEVISLPKNDLLQRLDALVAEEHLTVDARVYSYALALKHANAK
PFEVPFLKF