Ribose-5-phosphate Analysis Service

About Ribose-5-phosphate

Ribose 5-phosphate is the raw material for purine nucleotide synthesis. It can be generated either by the pentose phosphate pathway or by the reverse reaction of the aforementioned group transfer reaction of fructose 6-phosphate and glyceraldehyde 3-phosphate, which are intermediate products of sugar catabolism. But in the human body, it is mainly produced by the previous process.

Its chemical formula is C5H11O8P, and CAS number is 4300-28-1.

MS-based technical means can realize the qualitative and quantitative analysis of ribose-5-phosphate.

Molecular structure of ribose-5-phosphate

Technical Route of Targeted Metabolomics of Ribose-5-phosphate

Sample Requirements

Sample type

Plants, yeasts, microorganisms, etc.
Serum, plasma, urine, bile, bile acid, etc.
Cells, liver, brain tissue and other animal tissues and feces, etc.
There is no restriction on plant varieties. For varieties with special requirements or rare varieties, please contact our staff for more information.

Sample demand

Plant root / stem / leaf / fruit / seed > 200 mg fresh weight, freeze-dried sample > 0.5 g
Cells / microorganisms / cell supernatant / culture medium supernatant: the number of cells or bacteria > 10⁷, supernatant > 2 ml
Serum / plasma / urine / ruminal fluid / cerebrospinal fluid / amniotic fluid and other homogeneous liquid samples > 200 µl.
(Hemolysis should be avoided. Cerebrospinal fluid can be as low as 100 µl.)
Animal tissue / feces related samples > 200mg / sample
For other sample types, please consult technical support or sales.

Sample mixed and repeat

In order to ensure the accuracy of the samples and reduce systematic errors during sampling, it is necessary to select more than 3 materials with the same condition for each sample.
※ The same condition refers to the same period, basically the same phenotype, and the same part.
建议准备more than 6 biological replicates.

Storage and transportation

用液氮速冻保存即可minimize the leakage time of plant samples at room temperature, and store it at -80°C.
Dry ice transportation (about 3~4 kg dry ice is consumed every day, please use sufficient dry ice for transportation).

Feedback to Customers

Experimental steps
Related mass spectrometry parameters
Part of the mass spectrum picture
Raw data
Metabolic molecular identification results

Creative Proteomics offers several approaches to metabolomics studies, delivers precise and detailed data and analysis report. We can also customize the methods or establish new methods together with our collaborators, so they are fit-for-purpose and meet your specific needs. If you have any questions or specific requirements, please feel free tocontact us.

References

Kim M J, Lee M Y, Shon J C,et al. Untargeted and targeted metabolomics analyses of blackberries – Understanding postharvest red drupelet disorder.Food Chemistry, 2019, 300:125169.
Pawlak M, Klupczynska A, Kokot Z J,et al. Extending Metabolomic Studies of Apis mellifera Venom: LC-MS-Based Targeted Analysis of Organic Acids.Toxins, 2019, 12(1):14.
Wang X, Zhao X, Zhao J,et al. Serum metabolite signatures of epithelial ovarian cancer based on targeted metabolomics.Clinica Chimica Acta, 2021, 518: 59-69.